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vDAQ Acquisition System

The vDAQ acquisition system consists of the PCIe card installed in the PC and a breakout accessory connected by two cables to the PCIe card. The connector plate of the PCIe card (rear of PC) contains 4 high speed signal inputs for PMT signals and clock input/output terminals. The breakout has an analog input section at the top left with 12 inputs, an analog output section at the bottom left with 12 outputs, and a digital section at the right. The digital section is divided into 4 logical groups with 8 lines in each group.

Rear of PC:

Breakout:

Microscope Wiring

The vDAQ system is flexible in how microscope systems are wired up. Signals can be connected to any channel of the appropriate type. The different types of control signals are listed below based on the appropriate channel type. PMT signals attach directly to the back of the PCIe card using SMB cables.

Analog Outputs

SignalDescription
Galvo Position ControlEach galvo scanner in the system will have a position control signal
Resonant Zoom ControlScan amplitude control for resonant scanners
Beam Power ControlControl signal for pockels cells, or AOMs
Fast Focus ControlPiezo scanners, remote focus mirror, ETLs
PMT Gain ControlSome PMT controllers accept an analog signal to control the PMT gain

 

Analog Inputs

SignalDescription
Galvo Position FeedbackSome galvo controllers provide a feedback signal to measure the galvo position in real-time
Beam Power FeedbackBeam power signal, typically supplied from a photodiode measuring from a beam pick-off
Fast Focus FeedbackSome fast focus devices (such as piezos) provide a feedback signal to measure position in real-time

 

Digital Inputs

Digital inputs can be connected to any line in digital groups 0, 1 and 2.

SignalDescription
Resonant Sync (Period Trigger)Provided by resonant mirror driver as critical synchronization signal used for image formation.
Start TriggerA TTL transition will cause a running Acquisition Mode to start it's first Acquisition.
Next File TriggerA TTL transition will cause a LOOP to start the next Acquisition.
Stop TriggerA TTL transition will cause a running Acquisition to end.
PMT Trip StatusSome PMT controllers provide a TTL signal to indicate a "tripped" condition
Aux TriggersDigital inputs to timestamp
I2C Clock/DataClock (SCL) and data (SDA) lines for recording I2C messages (note, SDA must be wired to group 0 or 1 in order for "ack" output to be enabled)


Digital Outputs

Digital inputs can be connected to any line in digital groups 0, 1 and 3.

SignalDescription
Resonant Scanner EnableSome resonant scan systems accept a TTL signal to enable the resonant scanner
Shutter ControlSome shutters accept a TTL to control shutter position
PMT Power ControlSome PMT Controllers accept a TTL signal to activate the PMT
PMT Trip ResetSome PMT Controllers accept a TTL signal to clear a "tripped" condition
Imaging Clock Signals

ScanImage can output various clock signals which can be useful for synchronizing other equipment to imaging. Available clock signals include:

Pixel clock, line clock, beam clock, frame clock, and volume clock

 

 

Example Wiring Configuration

The table below lists signal connections for a dual-path microscope with one RGG scan path with remote focus, one GG scan path with ETL, one pockels cell on each path, and one shutter on each path

SignalChannel
Scanner 1 X Galvo ControlAO0
Scanner 1 Y Galvo ControlAO1
Scanner 1 X Galvo FeedbackAI0
Scanner 1 Y Galvo FeedbackAI1
Scanner 1 Resonant Zoom ControlAO2
Scanner 1 Resonant Enable ControlD3.0
Scanner 1 Resonant SyncD2.0
Scanner 1 Pockels ControlAO3
Scanner 1 Calibration PhotodiodeAI3
Scanner 1 Remote Focus ControlAO4
Scanner 1 Remote Focus FeedbackAI4
Scanner 1 ShutterD3.1
Scanner 2 X Galvo ControlAO5
Scanner 2 Y Galvo ControlAO6
Scanner 2 X Galvo FeedbackAI5
Scanner 2 Y Galvo FeedbackAI6
Scanner 2 Pockels ControlAO7
Scanner 2 Calibration PhotodiodeAI7
Scanner 2 ETL ControlAO8
Scanner 2 ShutterD3.2