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The ResScan Imaging System is used when a Resonant Scanning System resonant scanning system (or Vidrio Technologies RMR) is configured and NI FlexRIO hardware is selected. The screenshot below shows an example of this configuration in the Machine Configuration Editor (accessed by selecting the Machine Configuration... option in the File Menu on the Main Controls panel).

 

ResScan Acquisition System

The ResScan acquisition system is the minimum hardware required for raster image scanning.

It consists of a PXIe chassis which is connected to a PC via a PCI Express card.  The PXIe chassis has at least two cards installed:

 

For Thorlabs systems, see Thorlabs Scope Wiring below

 

For all other systems, connect according to the schematic at right:

  • Connect lines from PMT amplifiers to the analog inputs on the digitizer module.
  • Connect the resonant mirror's Period Trigger to PFI0 on the breakout for the scan control DAQ.
  • Connect the control line for the X Zoom to an analog output on the breakout for the scan control DAQ.
  • Connect the control line for the Y Mirror to an analog output on the breakout for the scan control DAQ.
  • Connect any necessary Acquisition Triggering (Start, Next, Loop) to PFI1-4.  These connections are configured using the Triggers Contol.
  • To configure a Shutter for ResScan, review the article Shutter Configuration
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Beams
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BeamsWiring

The Beams subsystem requires its own DAQ module.  There are two supported configurations.

For both:

  • connect calibration inputs to the desired analog input terminals.  
  • connect control lines to the desired analog outputs.

PXI-based

The relevant timing signals are communicated through the PXI backplane; no extra wiring is needed.

PCIe-based

Connect PFI5 of the scan control DAQ breakout box to the PFI terminal on the Beam DAQ board.

The settings for the routing of triggers are accessed in the Power Modulation (Beams) section of the ScanImage Machine Configuration Editor.

In this case, select "PFI0" for the Line Clock Input Terminal:

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Note

The Pockels Cell (Beams) control task is a timed Daqmx Task. Due to Daqmx limitations on timing resources this requires the Pockels Cell to run on a Daq card by itself. Other timed tasks such as Galvo control or FastZ piezo control must run on separate Daq boards.

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Fast Z Concepts

The Fast Z subsystem requires its own DAQ module.  There are two supported configurations.

For both: 

  • connect the control line to the desired analog output.

PXI-based

The relevant timing signals are communicated through the PXI backplane; no extra wiring is needed.

PCIe-based

Connect PFI6 of the scan control DAQ breakout box to the PFI terminal on the FastZ DAQ board.

The settings for the automatic routing of the frame clock trigger for Fast Z is accessed in the FastZ Actuator Setup section of the ScanImage Machine Configuration Editor.

In this case, select "PFI0" for the Frame Clock Input Terminal:

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Note

The FastZ Piezo control task is a timed Daqmx Task. Due to Daqmx limitations on timing resources this requires the FastZ Piezo to run on a Daq card by itself. Other timed tasks such as Galvo control or Pockels Cell control control must run on separate Daq boards.

 

 

 

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Signals Summary

Timing Signals  (DigitalIODevice)

Inputs (to DigitalIODeviceName)

SignalDescriptionDAQ Terminal
Period TriggerProvided by resonant mirror driver as critical synchronization signal used for image formation.PFI 0
Start TriggerA TTL transition will cause a running Acquisition Mode to start it's first Acquisition.Specifed in Triggers
Next File TriggerA TTL transition will cause a LOOP to start the next Acquisition.Specifed in Triggers 
Stop TriggerA TTL transition will cause a running Acquisition to end.Specifed in Triggers 

Outputs (from DigitalIODeviceName)

SignalDescription

DAQ Terminal

Beam ClockA TTL pulse generated on each line that controls when waveforms driving the Pockels cells begin.PFI 5

Frame Clock

A TTL pulse generated at the start of each frame.PFI 6
Acq Trigger

A TTL pulse generated when an acquisition starts. Useful for responding to software-triggered acquisitions.

PFI 7

Analog Inputs

SignalDescription
Channels 1-4Voltage inputs used for imaging, typically from the PMTs.
BeamsVoltage from a photodiode reporting the transmission through a Pockels cell. For calibration. Each Pockels cell requires an analog input channel.

Analog Outputs

SignalDescription
X ZoomControl voltage specifying the scan amplitude of the resonant mirror.
Y MirrorControl voltage specifying the deflection of the Y mirror.
BeamsControl voltage specifying for controlling a Pockels cell. Each Pockels cell requires one analog output channel.
Fast Z ConceptsControl voltage specifying the deflection of an objective positioner used for fast volume imaging.

 

Thorlabs Scope Wiring 

Include Page
Thorlabs Scope Wiring
Thorlabs Scope Wiring


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