General Information
ScanImage 2015a is software for resonant two-photon laser-scanning microscopy.
ScanImage supports numerous microscope platforms and is built on the same flexible acquisition hardware as our public release ScanImage 5.
ScanImage 2015a adds several premium features atop of ScanImage 5:
- targeted photostimulation (with parameteric curves, e.g. spirals & rasters)
- parallel scanning for simultaneous imaging & photostimulation, with image registration alignment
- multi region-of-interest (MROI) imaging
- step & settle FastZ mode, e.g. suited for Optotune electrically tunable lenses
- auxiliary triggers for timestamping external events (e.g. behavior events)
- syncing of acquisition to laser clock
- semi-automated bidirectional scan alignment
See ScanImage Licensing for pricing info.
Contact scanimage@vidriotech.com for a quotation or any questions.
Upgrading Information
Unless specifically noted (as indicated by a compatibility note), upgrading from an older release of ScanImage 2015 to a newer build will not break compatibility with Machine Data Files, User files, Configuration files, or TIFF headers. ScanImage can be upgraded by deleting the contents of the scanimage installation directory and copying in the new contents of the zip download. It may be useful to keep a backup of the old installation directory.
Version Notes
March Release (10 March 2016)
- Support for NI-DAQmx 15.5
- Support for logging when imaging multiple ROIs in LinScan
- Several bugfixes and performance improvements in LinScan
- Fixes for openTif
January Release (22 January 2016)
- Compatibility Note for Thorlabs BScope 2 users
- The MDF entries for the BScope2 component changed. The following link has instructions to modify your configuration for the new version:
ScanImage January 2016 Release Thorlabs BScope 2 Compatibility Information
- The MDF entries for the BScope2 component changed. The following link has instructions to modify your configuration for the new version:
- Support for Thorlabs MCM3000, USB PMT2100, USB BCM
- Support for higher sampling rates on certain DAQs in LinScan
- Fix powerbox with P-Z adjustment
- Improve performance of opentif
- Improved automatic scan phase adjustment for ResScan
- Automatic scan phase adjustment for LinScan
- New NI VISA interface
- Fix several bugs in LinScan
November Release (18 November 2015)
Powerbox
- Cycle mode
- Striped acquisition in LinScan (Galvo-Galvo scanning)
Fixes to LinScan (Galvo-Galvo scanning) when using a PCI-6110
- Fixes to LinScan (Galvo-Galvo scanning) when performing a Z-stack acquisition
- Performance enhancement for openTiff
August Release (11 August 2015)
Performance enhancements to acquisition starting and photostimulation starting
Performance enhancements to loading of usr and cfg files
Option to keep resonant scanner on when no acquisition is active
Improved mapping of ROIs from cell picker interface
Automatic sensor calibration and max/min position limits for purse analog controller
Support for mulitple linear scan paths
Support for photon counting modules
Improved linear scanning waveform with settling within fill fraction
Support for DAQmx 15.0.x
Remove TIFF header and footer size limitations
Fix centered z stack functionality with fastZ
Fix errors in slow stacks with some motors
Fix panning channel display in Matlab 2014b and newer
Fix scroll wheel functionality in Matlab 2014b and newer
Fix reference leaks with ROI group, ROI, and scanfield objects
Fix issue where footer is only attached to last TIFF in acquisition
Fix loading and storing of photostim monitor calibration
- Compatibility Note: A new section for photostim will be created in the MDF. Analog input channels for
monitoring are now stored here instead of in cfg file.
June Release (26 June 2015)
- Updated Thor B-Scope2 support for new stage controller firmware
- Panning, scroll wheel zoom, and point-to-point alignment in alignment gui
- Automatic photostim monitor calibration
- Improved stimulus editor functionality
- Fix issue causing acquisition to randomly stop with a data corruption error
- Fix for Thor ECU1 component trigger routing
- Fix photostim shuttering functionality
- Fix photostim monitoring display behavior during focus
- Fix to multiple issues after switching between imaging systems
- Fix to AO generation when resonant angular range is different from x galvo angular range
- Fix issue when importing cell picker rois in linear scanning mode
- Fix to stimulus roi constraints
- Fix loading of channels in opentif
May Release (31 May 2015)
First release of ScanImage 2015! New features including:
- Multiple region of interest support
- Linear scanning
- Parallel scanning
- Photostim
- Improved stability
- Many more!