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General Information

ScanImage 2015a is software for resonant two-photon laser-scanning microscopy.  

ScanImage supports numerous microscope platforms and is built on the same flexible acquisition hardware as our public release ScanImage 5.

ScanImage 2015a adds several premium features atop of ScanImage 5:

See ScanImage Licensing for pricing info.

Contact scanimage@vidriotech.com for a quotation or any questions.

Upgrading Information

Unless specifically noted (as indicated by a compatibility note), upgrading from an older release of ScanImage 2015 to a newer build will not break compatibility with Machine Data Files, User files, Configuration files, or TIFF headers. ScanImage can be upgraded by deleting the contents of the scanimage installation directory and copying in the new contents of the zip download. It may be useful to keep a backup of the old installation directory.


Prior to the August 2015 release of ScanImage 2015, ScanImage stored some persistent calibration and configuration info (items including line phase adjustment and scanner-to-scanner alignment) within the ScanImage directory. When upgrading to a new release of ScanImage, these files should be preserved. Starting with the August release, a location outside the installation directory can be specified to make upgrading ScanImage and backing up the complete configuration easier. If you are using a Machine Data File created before the August release, ScanImage will continue to save this info in the installation directory. It is highly recommended to move the saving location. You can do so with the following steps:

  1. Add the following code to the first (%% ScanImage) section of your Machine Data File:

    The default setting will store the files where the MDF is saved in a sub directory called ConfigData.

  2. Move the old configuration data to the new location by copying the files are listed below to the new directory. Your particular installation may or may not include each of these files:
    1. [ScanImage Installation Directory]\+scanimage\+components\+scan2d\private\ResScan_classData.mat
    2. [ScanImage Installation Directory]\+scanimage\+components\+scan2d\private\LinScan_classData.mat
    3. [ScanImage Installation Directory]\+scanimage\+components\private\Photostim_classData.mat

Version Notes

March Release (10 March 2016)

  • Support for NI-DAQmx 15.5
  • Support for logging when imaging multiple ROIs in LinScan
  • Several bugfixes and performance improvements in LinScan
  • Fixes for openTif

January Release (22 January 2016)

  • Compatibility Note for Thorlabs BScope 2 users
  • Support for Thorlabs MCM3000, USB PMT2100, USB BCM
  • Support for higher sampling rates on certain DAQs in LinScan
  • Fix powerbox with P-Z adjustment
  • Improve performance of opentif
  • Improved automatic scan phase adjustment for ResScan
  • Automatic scan phase adjustment for LinScan
  • New NI VISA interface
  • Fix several bugs in LinScan

November Release (18 November 2015)

  • Powerbox

  • Cycle mode
  • Striped acquisition in LinScan (Galvo-Galvo scanning)
  • Fixes to LinScan (Galvo-Galvo scanning) when using a PCI-6110

  • Fixes to LinScan (Galvo-Galvo scanning) when performing a Z-stack acquisition
  • Performance enhancement for openTiff

August Release (11 August 2015)

  • Performance enhancements to acquisition starting and photostimulation starting

  • Performance enhancements to loading of usr and cfg files

  • Option to keep resonant scanner on when no acquisition is active

  • Improved mapping of ROIs from cell picker interface

  • Automatic sensor calibration and max/min position limits for purse analog controller

  • Support for mulitple linear scan paths

  • Support for photon counting modules

  • Improved linear scanning waveform with settling within fill fraction

  • Support for DAQmx 15.0.x

  • Remove TIFF header and footer size limitations

  • Fix centered z stack functionality with fastZ

  • Fix errors in slow stacks with some motors

  • Fix panning channel display in Matlab 2014b and newer

  • Fix scroll wheel functionality in Matlab 2014b and newer

  • Fix reference leaks with ROI group, ROI, and scanfield objects

  • Fix issue where footer is only attached to last TIFF in acquisition

  • Fix loading and storing of photostim monitor calibration

  • Compatibility Note: A new section for photostim will be created in the MDF. Analog input channels for
    monitoring are now stored here instead of in cfg file. 

June Release (26 June 2015)

  • Updated Thor B-Scope2 support for new stage controller firmware
  • Panning, scroll wheel zoom, and point-to-point alignment in alignment gui
  • Automatic photostim monitor calibration
  • Improved stimulus editor functionality
  • Fix issue causing acquisition to randomly stop with a data corruption error
  • Fix for Thor ECU1 component trigger routing
  • Fix photostim shuttering functionality
  • Fix photostim monitoring display behavior during focus
  • Fix to multiple issues after switching between imaging systems
  • Fix to AO generation when resonant angular range is different from x galvo angular range
  • Fix issue when importing cell picker rois in linear scanning mode
  • Fix to stimulus roi constraints
  • Fix loading of channels in opentif

May Release (31 May 2015)
First release of ScanImage 2015! New features including:

  • Multiple region of interest support
  • Linear scanning
  • Parallel scanning
  • Photostim
  • Improved stability
  • Many more!


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