ScanImage 5.1 supports a wide variety of scanning systems including linear (galvo/galvo, GG) and resonant/galvo (RG) systems.
Supported scanning configurations can be ordered as a preassembled system, or as individual components.
Cambridge Technology & others
Acquisition and Control Hardware
ScanImage 5.1 allows users to configure their system for resonant scanning or linear scanning (=galvo only scanning). If you are using ScanImage 2015, a microscope with two laser paths can combine both methods to allow for simultaneous imaging and photostimulation.
The sections below describe the basic acquisition and control hardware requirements for both imaging systems.
ScanImage uses the National Instruments National Intruments FlexRIO platform for the core acquisition and control tasks for resonant two-photon laser scanning microscopy
ScanImage supports a handful of pre-specified FlexRIO system configurations
|Chassis/controller||PXIe-1073 or better|
|Scan Control DAQ||PXIe-6341||NI PXI-based X Series DAQ|
NI Digitizer Module
NI PXI-based FlexRIO Module
For SI5 - there will be no difference in operation. Most users should order the PXIe-7961R.
Future versions may utilize the far greater (~10x) processing power of the PXIe-7975R.
Additional X Series multifunction DAQ boards for some features:
ScanImage uses National Instruments Multifunction DAQ boards for image acquisition and microscope control.
PMT acquisition, galvo control
|For multi-PMT acquisition, a simultaneously sampled NI X-series board is required.|
|Pockels control||PCIe/PXIe-6341||Highly recommended - allows blanking laser during resonant scanner slowdown and galvo scanner flyback|
|Piezo/FastZ control||PCIe/PXIe-6341||Allows for volume imaging applications|
Photomultiplier tubes require a transimpedance amplifier for recording by ScanImage.
The optimal bandwidth depends on the sample rate and shortest pixel durations.
The Femto DHCPA-100 is a commercial off-the-shelf option suited for ScanImage 5 and later, at its 80MHz setting.
Thorlabs also makes a usable transimpedance amplifier. Please contact them directly for a part number and quote.
64-bit processor with at least 4 cores, ≥ 2GHz
Processors/systems with at least 6 cores may be valuable for future ScanImage or user-specific requirements combining TPLSM imaging with other simultaneous processing/acquisition
≥ 8 GB RAM
Additional RAM may be valuable for future ScanImage or user-specific requirements, e.g. online image analysis done in User Functions
Additional PCIe slots may be valuable for future ScanImage or other user-specific requirements
A separate drive or partition for OS and data logging/storage is recommended, to avoid contention
Either a RAID 0 magnetic drive pair or solid-state drive (SSD), whole or partition, is recommended for data logging/storage, to achieve high performance.
Piezo Hardware (Optional)
|Physik Instrumente||E-665||PIFOC piezos (e.g. P-721, P-725, P-725.xDD, P-725KHDS)|
|nPoint||LC.40x||nPFocus objectives||Options from 100um to 1000um of travel|