Skip to end of metadata
Go to start of metadata
Contents

Scanhead Hardware

SI5 achieves fast raster scanned images using control of two scanners/mirrors: one resonant and one galvo scanner

This resonant/galvo (RG) pair can be ordered as a preassembled system, or as individual components.

Acquisition and Control Hardware

ScanImage 5 uses the National Instruments FlexRIO platform for the core acquisition and control tasks for resonant two-photon laser scanning microscopy

SI5 supports a handful of pre-specified FlexRIO system configurations

NI ScanImage 5 Bundle

Icon

Vidrio Technologies offers National Instruments hardware bundles, to help users create valid configurations. If you also order a ScanImage optimized computer from us at the same time, Vidrio will install ScanImage and create a custom machine data file for your system.

Email us at support@vidriotech.com for a ScanImage hardware bundle quote.

(info) See notes below for further information about the key options

Required

RoleItem(s)Performance characteristics
Chassis/controllerPXIe-1073 
Scan Control DAQPXIe-6341NI PXI-based X Series DAQ
Digitizer

One of

NI Digitizer Module

  • 2 channel
  • 4 channel
FPGA Module

PXIe-7961R

NI PXI-based FlexRIO Module. The PXIe-7975R is also supported, but not typically recommended. Contact us if any questions.

Optional

Additional X Series multifunction DAQ boards for some features:

PurposeOptionsNotes
Pockels controlPCIe-6321 or PXIe-6341Highly recommended - allows blanking laser during resonant scanner slowdown and galvo scanner flyback
Piezo/Fast Z controlPCIe-6321 or PXIe-6341Allows for volume imaging applications
Icon

The PXIe option is moderately more expensive, but has some advantages:

  1. Simplifies wiring complexity between boards.
  2. Reduces the number of PCIe slots required in the computer (or makes more available).

 

Vidrio Technologies offers National Instruments hardware bundles, to help users create valid configurations. If you also order a ScanImage optimized computer from us at the same time, Vidrio will install ScanImage and create a custom machine data file for your system.

Email us at support@vidriotech.com for a ScanImage hardware bundle quote.

(info) See notes below for further information about the key options

Preamplifier Hardware

Photomultiplier tubes require a transimpedance amplifier for recording by ScanImage.The optimal bandwidth depends on the sample rate and shortest pixel durations.  

For resonant scanning, we recommend a bandwidth of at least 30 Mhz.

Two recommended options are:

VendorPart NumberNotes
ThorlabsTIA60
  • fixed gain & bandwidth (30,000 V/A & 60 MHz)
  • cost-effective
FemtoDHCPA-100
  • variable gain & bandwidth (up to 80 MHz at 10^4 gain)

(tick) Most users will likely prefer the more cost-effective TIA60

Computer Hardware - Ask Us about a Custom Computer Build with ScanImage Preinstalled and Tested

 

 Recommendation
CPU

64-bit processor with at least 4 cores, ≥ 3GHz.

Examples:

  • i7-4790K 4GHz Devil's Canyon processor tops the benchmark list

(info) Processors/systems with at least 6 cores may be valuable for future ScanImage or user-specific requirements combining TPLSM imaging with other simultaneous processing/acquisition

RAM

≥ 16 GB RAM

(info) Additional RAM may be valuable for future ScanImage or user-specific requirements, e.g. online image analysis done in User Functions 

PCIe slots
  • PCIe x16 for a graphics card - Graphics card is not required if using the i7-4790K as it has on board graphics which are fine for ScanImage operations
  • (required) PCIe x1 for MXI controller connecting to the PXI chassis
  • (optional) PCIe x1 for a PCIe-6321 dedicated to Pockels control
  • (optional) PCIe x1 for a PCIe-6321 dedicated to Piezo/Fast Z control.

(info) We recommend PXIe based DAQ's (e.g. PXIe-6341) for Beams and Fast Z features, which reduces the PCIe slot requirements accordingly. Only the PCIe x1 slot for the MXI controller card is strictly required.

(info) Additional PCIe slots may be valuable for future ScanImage or other user-specific requirements

(info) If other graphics intensive applications are going to be used on the computer then a graphics card may be required. We recommend a GTX 960.

Hard Drives

Recommend separate drives for OS and data logging/storage, to avoid contention and achieve maximum logging performance.

Examples:

  • SSD Drive for OS plus separate SSD for data logging/storage

OR

  • SSD Drive for OS plus separate magnetic RAID 0 drive pair
OS
  • Windows 7 Professional

 

Piezo Hardware (Optional)

VendorControllerCompatible PiezosComments
Physik InstrumenteE-665PIFOC piezos (e.g. P-721, P-725, P-725.xDD, P-725KHDS) 
nPointLC.40xnPFocus objectivesOptions from 100um to 1000um of travel