ScanImage supports a wide variety of scanning systems including linear (galvo/galvo, GG) and resonant/galvo (RG) systems. Resonant scanning with multiple
regions of interest (MROI) is supported for GG and resonant/galvo/galvo (RGG) systems. Parallel scanning systems are supported to provide simultaneous imaging
Supported scanning configurations can be ordered as a preassembled system, or as individual components.
Cambridge Technology & others
Acquisition and Control Hardware
ScanImage 2015 and later allows users to configure their system for resonant scanning or linear scanning (=galvo only scanning). A microscope with two laser paths can combine both methods to allow for simultaneous imaging and photostimulation.
The sections below describe the basic acquisition and control hardware requirements for both imaging systems.
ScanImage uses vDAQ for data acquisition and microscope control. vDAQ is an all-in-one data acquisition board that controls the most advanced laser scanning microscopes.
For linear Scanning, ScanImage supports two hardware platforms
- vDAQ (recommended)
- NI DAQ hardware (low cost)
vDAQ for linear scanning
See vDAQ Configuration for more information.
NI hardware for linear scanning
PMT acquisition, galvo control
|For multi-PMT acquisition, a simultaneously sampled NI X-series board is required.|
|Pockels control||PCIe-6321 OR PXIe-6341||Highly recommended - allows blanking laser during resonant scanner slowdown and galvo scanner flyback|
|Piezo/FastZ control||PCIe-6321 OR PXIe-6341||Allows for volume imaging applications|
Photomultiplier tubes require a transimpedance amplifier for recording by ScanImage.
The optimal bandwidth depends on the sample rate and shortest pixel durations.
The Femto DHCPA-100 is a commercial off-the-shelf option suited for ScanImage 5 and later, at its 80MHz setting.
Thorlabs also makes a usable transimpedance amplifier. Please contact them directly for a part number and quote.
64-bit processor with 4 cores, ≥ 3GHz
Processors/systems with at least 6 cores may be valuable for future ScanImage or user-specific requirements combining TPLSM imaging with other simultaneous processing/acquisition
16 GB RAM
Additional RAM may be valuable for future ScanImage or user-specific requirements, e.g. online image analysis done in User Functions
If PCIe based DAQ's (e.g. PCIe-6341) are used for Beams and Fast Z features, then additional PCIe slots will be required. Only the PCIe x1 slot for the MXI controller card is strictly required when using a PXIe chassis.
Additional PCIe slots may be valuable for future ScanImage or other user-specific requirements
A separate drive or partition for OS and data logging/storage is recommended, to avoid contention.
Either a RAID 0 magnetic drive pair or solid-state drive (SSD), whole or partition, is recommended for data logging/storage, to achieve write performance >300Mb/second.
Piezo Hardware (Optional)
|Physik Instrumente||PIFOC piezos (e.g. P-721, P-725, P-725.xDD, P-725KHDS)|
|nPoint||LC.40x||nPFocus objectives||Options from 100um to 1000um of travel|
|Generic||Any focusing device accepting a -10V..10V analog command signal.|
Stage Hardware (Optional)
|Vendor||Stage Controllers||SI Free||SI Premium|
|Tholabs||MCM3000, MCM5000, Bergamo II Scope|
|Physik Instrumente||E-665, E-709 (CHG), E-753, C-884, C-863|
|Luigs & Neumann||SM5, SM7|
*Requires MATLAB Instrument Control Toolbox
SLM Hardware (Optional)
|Meadowlark Optics||PCIe8 (ODP series)|
|Meadowlark Optics||1920x1152 SLM|
|-||DVI/HDMI controlled SLM|