Bruker Microscope Wiring
ScanImage supports communication with the Bruker Resonant Controller and the Bruker MAMC stage controller. ScanImage is an open-source software package for controlling laser scanning microscope with a comprehensive feature list. It is written in Matlab and is fully scriptable and customizeable by the user. Please contact firstname.lastname@example.org for more information.
The following connections need to be made to connect a Bruker resonant scanning system to ScanImage:
|Signal Source||Signal Sink||Description|
|Bruker Resonant Controller / RS-232||SI Computer COM Port||Controls Resonant Scanner Amplitude|
|Bruker Resonant Controller / Sync (MMCX)||SI Primary DAQ board / PFI0||Resonant Scanner Sync Signal|
|SI Primary DAQ board / PFI11||Bruker Resonant Controller / Trigger In||Starts the generation of the resonant scanner sync signal|
|SI Primary DAQ board / AO0||Bruker Galvo Controller / X Input||X-Galvo Position Control|
|SI Primary DAQ board / AO1||Bruker Galvo Controller / Y input||Y-Galvo Position Control|
|Bruker Galvo Controller / X Feedback||SI Primary DAQ board / AI0||X-Galvo Position Feedback|
|Bruker Galvo Controller / Y Feedback||SI Primary DAQ board / AI1||Y-Galvo Position Feedback|
|disconnected / grounded||Bruker Galvo Controller / Switch||Switches between X1/Y1 and X2/Y2 input|
Start ScanImage and create a new Machine Data File. In the ScanImage Machine Configuration Editor, add an entry for a Resonant Scanner and a Linear Scanner. In the Additional Components and Plugins section, add an entry for 'Bruker Resonant Controller'.
The resonant scanner amplitude is set through a serial connection, and does not require an analog output. Set the analog output to SI Primary DAQ board / AO0. This is just a placeholder value and is not going to be used.
Configure the Bruker Resonant Control Plugin: enter the resonant scanner name from step 1 to link the plugin to the scanner. Specify the DAQ device and trigger terminal to send the start trigger to the Bruker Resonant Control Box.
ScanImage currently does not support the configuration of the Bruker PMT Preamplifier. Use Prairie View to set appropriate Parameters. These settings persist when Prairie View is closed.
Note: for resonant scanning, filter settings of 15MHz or higher are appropriate. When using linear (galvo/galvo) scanning with ScanImage, these settings do not need to be changed, since ScanImage samples the PMT with high speed even in linear scanning mode.
In the Machine Configuration Editor, add an entry for the Bruker MAMC stage controller: