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ScanImage 2015 and later allows users to configure their system for resonant scanning or linear scanning (=galvo only scanning). A microscope with two laser paths can combine both methods to allow for simultaneous imaging and photostimulation.

The sections below describe the basic acquisition and control hardware requirements for both imaging systems.

 

 

Resonant Scanning

ScanImage uses vDAQ for data acquisition and microscope control. vDAQ is an all-in-one data acquisition board that controls the most advanced laser scanning microscopes.

vDAQ PCIe

 

The 2U rackmountable vDAQ breakout shows the main functionality of the data acquisition system:

  • 4 fast analog inputs for acquiring PMT data
  • 12 2MHz analog outputs for microscope control (Galvos, Piezos, Pockels Cells, ...)
  • 12 1MHz analog inputs for actuator feedback
  • 32 digital inputs/outputs for triggering / clocking 

 

 

Linear Scanning

For linear Scanning, ScanImage supports two hardware platforms

  1. vDAQ (recommended)
  2. NI DAQ hardware (low cost)

 

vDAQ for linear scanning

See vDAQ Configuration for more information.

NI hardware for linear scanning

RoleItem(s)Notes

PMT acquisition, galvo control

PCIe-6374
OR
PXIe-6356

 For multi-PMT acquisition, a simultaneously sampled NI X-series board is required.

Optional

RoleItem(s)Notes
Pockels controlPCIe-6321 OR PXIe-6341Highly recommended - allows blanking laser during resonant scanner slowdown and galvo scanner flyback
Piezo/FastZ controlPCIe-6321 OR PXIe-6341Allows for volume imaging applications
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Users upgrading from ScanImage 3.8 can reuse their existing PCI-6110/6132/6133 digitizers, but will need to purchase at least one additional X-series board (e.g. PCIe-6321). Please contact us at support@vidriotech.com to learn about upgrade options from ScanImage 3.x

Configuration Guide

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Please see Configuring LinScan (Galvo Galvo scanning) page for additional details.

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